LOCUS       pLV-[hsa-miR-1226]        9128 bp    DNA     circular     2-MAR-2011
SOURCE      
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7049..7909
                     /label=Amp(R)
     promoter        8110..8650
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5654..5888
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6463..6918)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5960..6090
                     /label=SV40 pA
     misc_feature    6091..6304
                     /label=SV40 ori
     misc_feature    4975..5571
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3593
                     /label=intron
     CDS             3640..4965
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3383
                     /label=hsa-miR-1226 precursor
BASE COUNT     2160 a      2383 c      2492 g      2093 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gcagggcaag 
     3121 gtcacccggc aggggaagtt caagcccaac agcgtcacat ataggaccaa atcaggcaac 
     3181 atcctgctgc acaagtcgac cattaacagg tgagggcatg caggcctgga tggggcagct 
     3241 gggatggtcc aaaagggtgg cctcaccagc cctgtgttcc ctagggaggc cacacggtta 
     3301 cggagccgat ggctgacgta tttcatggca gtcaagtcca atggcagcgt cttcgtccgg 
     3361 gactcctctc aggtgcaccc gctgctagct cgagcttttg gagtacgtcg tctttaggtt 
     3421 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta 
     3481 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt 
     3541 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt 
     3601 gaaaactacc cctctagagt cgagctaccg gtcgccacca tggtgagcaa gggcgaggag 
     3661 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg 
     3721 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 
     3781 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc 
     3841 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 
     3901 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 
     3961 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg 
     4021 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc 
     4081 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag 
     4141 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag 
     4201 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc 
     4261 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc 
     4321 accggcggca tggacgagct gtacaaggac ccaccggtcg ccaccatgac cgagtacaag 
     4381 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 
     4441 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 
     4501 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 
     4561 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 
     4621 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 
     4681 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 
     4741 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 
     4801 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 
     4861 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgagtgcccg 
     4921 aaggaccgcg cgacctggtg catgacccgc aagcccggtg cctgagcggc cgcgtcgaca 
     4981 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 
     5041 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 
     5101 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 
     5161 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 
     5221 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 
     5281 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 
     5341 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 
     5401 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 
     5461 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 
     5521 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag 
     5581 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 
     5641 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 
     5701 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 
     5761 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 
     5821 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 
     5881 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 
     5941 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 
     6001 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
     6061 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 
     6121 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 
     6181 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 
     6241 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc 
     6301 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 
     6361 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 
     6421 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 
     6481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     6541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     6601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
     6661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     6721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     6781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
     6841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 
     6901 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     6961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     7021 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
     7081 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
     7141 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
     7201 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
     7261 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
     7321 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
     7381 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
     7441 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
     7501 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
     7561 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
     7621 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
     7681 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
     7741 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
     7801 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
     7861 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     7921 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     7981 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     8041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     8101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     8161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     8221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     8281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     8341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     8401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     8461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     8521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     8581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     8641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     8701 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 
     8761 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 
     8821 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 
     8881 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 
     8941 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 
     9001 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 
     9061 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 
     9121 ggagctgc 
//
